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Chin. Opt. Lett.
 Home  List of Issues    Issue 03 , Vol. 08 , 2010    10.3788/COL20100803.0278


Fourier analysis of elastic light scattering spectrum of epithelial cell nuclei
Qinghua Wang, Zhenhua Li, Jiancheng Lai, Anzhi He
Department of Information Physics and Engineering, [Nanjing University of Science and Technology], Nanjing 210094, China

Chin. Opt. Lett., 2010, 08(03): pp.278-281-4

DOI:10.3788/COL20100803.0278
Topic:Fourier optics and signal processing
Keywords(OCIS Code): 070.4790  290.5825  300.6300  

Abstract
A new method for simultaneously determining the size and refractive index of epithelial cell nuclei is presented. The function of the modified elastic light scattering spectrum is regarded as a function of wave number factor, Q=2\lambda<sup>-1</sup> sin(\theta/2). The modified spectrum has a constant oscillation period with its frequency proportional to the average diameter of cell nuclei. To the same average diameter, the different relative refractive indexes of epithelial cell nuclei only induce the horizontal shift of the spectra. Both the oscillation frequency and the horizontal shift are quantified by the fast Fourier transform on the modified spectra. The average diameter can be figured out through the peak frequency divided by the value of the refractive index of the surrounding medium. The phase angle of the peak frequency has an approximate linear relationship with the relative refractive index of epithelial cell nuclei.

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Received:2009/6/9
Accepted:
Posted online:

Get Citation: Qinghua Wang, Zhenhua Li, Jiancheng Lai, Anzhi He, "Fourier analysis of elastic light scattering spectrum of epithelial cell nuclei," Chin. Opt. Lett. 08(03), 278-281-4(2010)

Note: This work was supported by the National Natural Science Foundation of China (Nos. 10904072 and 10704039), the Startup Foundation (No. AB411994), and the Devel-opment Foundation from Nanjing University of Science and Technology (No. XKF09043).



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