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Chin. Opt. Lett.
 Home  List of Issues    Issue 03 , Vol. 08 , 2010    10.3788/COL20100803.0278

Fourier analysis of elastic light scattering spectrum of epithelial cell nuclei
Qinghua Wang, Zhenhua Li, Jiancheng Lai, Anzhi He
Department of Information Physics and Engineering, [Nanjing University of Science and Technology], Nanjing 210094, China

Chin. Opt. Lett., 2010, 08(03): pp.278-281-4

Topic:Fourier optics and signal processing
Keywords(OCIS Code): 070.4790  290.5825  300.6300  

A new method for simultaneously determining the size and refractive index of epithelial cell nuclei is presented. The function of the modified elastic light scattering spectrum is regarded as a function of wave number factor, Q=2\lambda<sup>-1</sup> sin(\theta/2). The modified spectrum has a constant oscillation period with its frequency proportional to the average diameter of cell nuclei. To the same average diameter, the different relative refractive indexes of epithelial cell nuclei only induce the horizontal shift of the spectra. Both the oscillation frequency and the horizontal shift are quantified by the fast Fourier transform on the modified spectra. The average diameter can be figured out through the peak frequency divided by the value of the refractive index of the surrounding medium. The phase angle of the peak frequency has an approximate linear relationship with the relative refractive index of epithelial cell nuclei.

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Get Citation: Qinghua Wang, Zhenhua Li, Jiancheng Lai, Anzhi He, "Fourier analysis of elastic light scattering spectrum of epithelial cell nuclei," Chin. Opt. Lett. 08(03), 278-281-4(2010)

Note: This work was supported by the National Natural Science Foundation of China (Nos. 10904072 and 10704039), the Startup Foundation (No. AB411994), and the Devel-opment Foundation from Nanjing University of Science and Technology (No. XKF09043).


1. V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, Nature 406, 35 (2000).

2. R. S. Gurjar, V. Backman, L. T. Perelman, I. Georgakoudi, K. Badizadegan, I. Itzkan, R. R. Dasari, and M. S. Feld, Nature Med. 7, 1245 (2001).

3. K. Sokolov, L. T. Nieman, A. Myakov, and A. Gillenwater, Tech. Canc. Res. Treat: 3, 1 (2004).

4. Y. Liu, X. Li, Y. L. Kim, and V. Backman, Opt. Lett. 30, 2445 (2005).

5. L. Xu, H. Li, and Y. Zheng, Chin. Opt. Lett. 7, 64 (2009).

6. Q. H. Wang, Z. H. Li, J. C. Lai, and A. Z. He, Chin.Phys. Lett. 24, 1076 (2007).

7. L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Mano haran, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, Phys. Rev. Lett. 80, 627 (1998).

8. C. W. Boone, G. D. Stoner, J. V. Bacus, V. Kagan, M. A. Morse, G. J. Kelloff, and J. W. Bacus, Canc. Epidemiol. Biomarkers Prev. 9, 495 (2000).

9. H. Qi, L. Ruan, S. Wang M. Shi, and H. Zhao, Chin. Opt. Lett. 6, 346 (2008).

10. H. Wang and J. Shen, Chin. Opt. Lett. 6, 871 (2008).

11. H. Jiang, G. Marquez, and L. V. Wang, Opt. Lett. 23, 394 (1998).

12. M. Bartlett and H. Jiang, Phys. Rev. E 65, 031906 (2002).

13. H. C. van de Hulst, Light Scattering by Small Particles (Dover Publications, New York, 1981) p.35.

14. W. J. Wiscombe, Appl. Opt. 19, 1505 (1980).

15. B. Beauvoit, T. Kitai, and B. Chance, Biophys. J. 67, 2501 (1994).

16. M. Kerker, W. A. Farone, L. B. Smith, and E. Matijevic, J. Colloid Sci. 19, 193 (1964).

17. C. M. Sorensen and D. Shi, J. Opt. Soc. Am. A 19, 122 (2002).

18. R. N. Bracewell, The Fourier Transform and Its Applications (McGraw Hill Companies, Singapore, 2000) p.5.

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